Photodynamic therapy is an effective modality for treating advanced melanoma. However, melanoma's inherent resistance to laser radiation hinders its widespread clinical application. The near-infrared laser radiation range of 1264-1270 nm offers unique properties: firstly, its ability to penetrate melanin-producing cells, and secondly, its capability to generate singlet oxygen without xenobiotics. We assess the impact of continuous wave 1265 nm laser radiation on an antioxidant defense system in melanoma B16-F10 and normal CHO-K1 cells. We observe a time-dependent increase in superoxide dismutase and glutathione-S-transferase activities, fluctuations in reduced glutathione levels, as well as a simultaneous increase in melanoma cell proliferation and cell death. We hypothesize that the differential activation of cellular antioxidant defense mechanisms contributes to melanoma cells' resilience to laser radiation.
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