Advances in structural studies of viruses by Raman spectroscopy

Stacy A. Towse; James M. Benevides; George J. Thomas Jr.

doi:10.1117/12.57327

1 May 1991 Advances in structural studies of viruses by Raman spectroscopy

Stacy A. Towse, James M. Benevides, George J. Thomas Jr.

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57327
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Assembly of an icosahedral capsid from a single species of coat protein subunit requires different subunit conformations at different lattice positions . In the double-stranded DNA bacteriophage P22 formation of correctly dimensioned capsids is mediated by interactions between subunits of coat and scaffolding proteins . We have employed Raman spectroscopy to investigate the specific intrasubunit conformations and intersubunit interactions required to close icosahedral shells which are competent to package the P22 genome . Preliminary results from coat protein subunits polymerized to form capsids in the presence and absence of the scaffolding protein indicate different distributions of subunit secondary structure for these two assembly conditions . The difference in structure affects a small portion of the coat subunit (z2 . 3 or 10 of 430 amino acid residues per subunit) and involves a transition from a-helix in the scaffoldassembled shell to B-strand in particles assembled without scaffold mediation . The secondary structure change is accompanied by changes in specific amino acid side chains indicative of a greater variety of side chain environments for particles assembled without scaffolding protein . The detection of small changes in protein structure is facilitated by recent developments in instrumentation and progress in the assignment of protein Raman bands to specific configurational states. Application of this methodology to the bacteriophage P22 tailspike protein has also permitted characterization of differences in thermal unfolding pathways of the wild-type protein and temperature-sensitive-folding mutant . Similar methods applied to mature icosahedral bacteriophages (P22 and TI) which package a double-stranded DNA chromosome reveal subtle but definitive perturbations to dsDNA conformation in the packaged state. 1.

Citation Download Citation

Stacy A. Towse, James M. Benevides, and George J. Thomas Jr. "Advances in structural studies of viruses by Raman spectroscopy", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57327

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Raman spectroscopy

Packaging

Picosecond phenomena

Thin film coatings

Crystals

Laser applications

Show All Keywords

Keywords/Phrases

Search In:

Publication Years