Selective protection of normal hepatocytes by indocyanine green in photodynamic therapy for the hepatoma of rat

Ying Gu M.D.; Junheng Li M.D.; Zhong-He Guo M.D.

doi:10.1117/12.137021

5 March 1993 Selective protection of normal hepatocytes by indocyanine green in photodynamic therapy for the hepatoma of rat

Ying Gu M.D., Junheng Li M.D., Zhong-He Guo M.D.

Author Affiliations +

Proceedings Volume 1616, International Conference on Photodynamic Therapy and Laser Medicine; (1993) https://doi.org/10.1117/12.137021
Event: International Conference on Photodynamic Therapy and Laser Medicine, 1991, Beijing, China

Abstract

Using hepatocarcinoma transplanted rats, the present study made consecutive observation for the color change and indocyanine green (ICG) absorption peak of the normal liver and tumor tissues after intravenous injection of ICG. The normal liver tissue of the rat was found to turn violet-green soon after ICG injection and the optic density (OD) of ICG-characteristic spectral peak of the tissue homogenate reached its maximum value at 35 minutes post-injection, while neither color change nor OD value increase was noticed in the tissue of transplanted hepatocarcinoma, suggesting that there is a specific absorption of ICG by the normal liver tissue. Chemiluminescentoassay revealed inhibited luminal chemiluminescence by ICG, indicating the depression of singlet oxygen and reactive oxygen species (ROS) oxidation during HPD photosensitization by ICG. In PDT of the hepatocarcinoma, the irradiated area was examined under microscope and auto-microimage analysis system after ICG administration. For tumor-free tissue, the photosensitization induced necrotic area was found smaller in those with than those without ICG administration, whereas the tumor killing effect was almost the same of the two. It is suggested that ICG may offer selective protection for healthy hepatocytes without diminishing the destruction of tumor cells. The protection of healthy hepatocytes by ICG is thought to be in accordance with the amount of ICG in the cell and the distribution of light energy.

Citation Download Citation

Ying Gu M.D., Junheng Li M.D., and Zhong-He Guo M.D. "Selective protection of normal hepatocytes by indocyanine green in photodynamic therapy for the hepatoma of rat", Proc. SPIE 1616, International Conference on Photodynamic Therapy and Laser Medicine, (5 March 1993); https://doi.org/10.1117/12.137021

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available