Stage-scanned chromatically aberrant confocal microscope for 3-D surface imaging

Mark Anthony Browne; Olusola Akinyemi; Francis Crossley; Duncan T. B. Stacey

doi:10.1117/12.59583

26 June 1992 Stage-scanned chromatically aberrant confocal microscope for 3-D surface imaging

Mark Anthony Browne, Olusola Akinyemi, Francis Crossley, Duncan T. B. Stacey

Proceedings Volume 1660, Biomedical Image Processing and Three-Dimensional Microscopy; (1992) https://doi.org/10.1117/12.59583
Event: SPIE/IS&T 1992 Symposium on Electronic Imaging: Science and Technology, 1992, San Jose, CA, United States

Abstract

A method for full-field surface profiling in the tandem scanning confocal microscope has been previously described. The technique utilizes chromatic aberration to produce an extended and color coded focal volume. Planes at different axial depths within this volume correspond to the foci of different wavelengths through the intentionally aberrant system. To determine the height of any point within the field, the confocal detection system must be capable of identifying the wavelength of the most intensely reflected light from that point. Hence the relative sensitivity of the detector and the spectral response of the imaging system are important parameters. We have utilized xenon and mercury arc sources and compared results with various types of objective lens. One potential limitation of this microscope is the fact that the introduction of longitudinal chromatic aberration affects the correction of the optics for plan imaging, i.e., introduces spherical aberrations, but the technique is rapid and produces results well correlated with more conventional techniques. To allow detailed study of the use of a chromatic 3-D probe for surface imaging we have designed and constructed a stage- scanned instrument which uses on-axis optics and is therefore free from spherical aberrations and can be corrected optimally. The instrument is described and results presented for single pass 3-D imaging.

Citation Download Citation

Mark Anthony Browne, Olusola Akinyemi, Francis Crossley, and Duncan T. B. Stacey "Stage-scanned chromatically aberrant confocal microscope for 3-D surface imaging", Proc. SPIE 1660, Biomedical Image Processing and Three-Dimensional Microscopy, (26 June 1992); https://doi.org/10.1117/12.59583

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microscopes

Confocal microscopy

3D image processing

Chromatic aberrations

Objectives

Image processing

Biomedical optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years