Spectral interferometric immunoassay with synthetic lipid-anchored polypeptide antigens

Andreas Brecht; Guenter Gauglitz; Werner Beck; Renate Spohn; Guenter Jung

doi:10.1117/12.143567

30 April 1993 Spectral interferometric immunoassay with synthetic lipid-anchored polypeptide antigens

Andreas Brecht, Guenter Gauglitz, Werner Beck, Renate Spohn, Guenter Jung

Proceedings Volume 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV; (1993) https://doi.org/10.1117/12.143567
Event: Fibers '92, 1992, Boston, MA, United States

Abstract

Spectral interferometric investigations, which were carried out at thin dielectric layers with a novel fixation procedure for synthetic antigens, are reported. This work is aimed at an optical, regenerable, label-free immunosensor for antibody determinations. Most optical biosensor approaches require fixation of some kind of receptor to the sensor surface. Simple adsorption leads to washout effects and often prohibits sensor regeneration. Covalent fixation usually decreases receptor affinity significantly. In this paper we report a noncovalent fixation procedure, based on synthetic, lipid anchored peptide antigens. Fixation is achieved by strong hydrophobic interaction between the pretreated sensor surface and a lipid anchor covalently linked to the antigen. Spectral interferometry uses short coherent (white) light interference for the determination of surface and volume effects at thin films. This method has been successfully applied to hydrocarbon sensing and solid phase adsorption immunoassay.

Citation Download Citation

Andreas Brecht, Guenter Gauglitz, Werner Beck, Renate Spohn, and Guenter Jung "Spectral interferometric immunoassay with synthetic lipid-anchored polypeptide antigens", Proc. SPIE 1796, Chemical, Biochemical, and Environmental Fiber Sensors IV, (30 April 1993); https://doi.org/10.1117/12.143567

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