Novel FIA chemiluminescence fiber optic biosensor for urinary and blood glucose

Maurice V. Cattaneo; J. H. T. Luong

doi:10.1117/12.144839

21 May 1993 Novel FIA chemiluminescence fiber optic biosensor for urinary and blood glucose

Maurice V. Cattaneo, J. H. T. Luong

Proceedings Volume 1886, Fiber Optic Sensors in Medical Diagnostics; (1993) https://doi.org/10.1117/12.144839
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A chemiluminescence fiber optic biosensor system coupled to FIA was developed to measure glucose in bodily fluids. Glucose oxidase was immobilized on a preactivated nylon membrane and attached to the tip of a fiber optic bundle. This enzyme acts on (beta) -D-glucose to produce hydrogen peroxide which was then reacted with luminol in the presence of ferricyanide to produce a light signal. The sensitivity of the biosensor was determined to be 32 +/- 0.65 nV (mu) M^-1 with a minimum detectable level of 5 (mu) M. The addition of a glucose oxidase column with a higher enzyme loading improved the sensitivity by at least 25-fold thus permitting the measurement of the lower glucose levels found in urine. The enzyme membrane could be reused for at least 50 analyses while the glucose oxidase column could be reused for over 500 analyses without losing the original activity. Endogenous ascorbate and urate usually present in urine samples which interfere with the chemiluminescence signal were effectively retained by an upstream ion exchange column. When applied for the determination of urinary and blood glucose levels, the results obtained compared well with those of the widely accepted hexokinase assay.

Citation Download Citation

Maurice V. Cattaneo and J. H. T. Luong "Novel FIA chemiluminescence fiber optic biosensor for urinary and blood glucose", Proc. SPIE 1886, Fiber Optic Sensors in Medical Diagnostics, (21 May 1993); https://doi.org/10.1117/12.144839

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available