NAD(P)H imaging of the cornea: functional imaging of the eye

Barry R. Masters

doi:10.1117/12.154961

17 September 1993 NAD(P)H imaging of the cornea: functional imaging of the eye

Barry R. Masters

Proceedings Volume 1894, Clinical Applications of Modern Imaging Technology; (1993) https://doi.org/10.1117/12.154961
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

This chapter describes the principles and practice of the optical recording of cellular metabolism. Redox imaging of cells, tissues, and organs based on intrinsic fluorescent probes of cellular metabolism. Cellular metabolism may be noninvasively interrogated through the `optical method' based on the fluorescence intensity of intrinsic probe molecules (Chance, 1991). The intrinsic fluorescent probes, which report on cellular metabolism, are the reduced pyridine nucleotides, NAD(P)H, and the oxidized flavoproteins.

Citation Download Citation

Barry R. Masters "NAD(P)H imaging of the cornea: functional imaging of the eye", Proc. SPIE 1894, Clinical Applications of Modern Imaging Technology, (17 September 1993); https://doi.org/10.1117/12.154961

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