Subcellular storage compartments of bacteriopheophorbide sensitizers

Joerg G. Moser; U. Dembeck; M. Hubert; Bernhard Spengler; Rainer Bayer; Birgit Wagner

doi:10.1117/12.168704

1 March 1994 Subcellular storage compartments of bacteriopheophorbide sensitizers

Joerg G. Moser, U. Dembeck, M. Hubert, Bernhard Spengler, Rainer Bayer, Birgit Wagner

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168704
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Fluorescence colocalization with the Golgi specific stain, NBD-ceramide, and the mitochondrial localizing stain, Rhodamine 123, confirmed the earlier assumption that the Golgi apparatus is one of the prominent storage compartments for bacteriopheophorbide esters in OAT 75 SCLC cells and several amelanotic melanoma cell lines (A375, Melur SP18, SkAMel 25). Furthermore, a diffuse staining of mitochondria, of non-structured cytoplasm, and an additional storage in melanine vesicles of the amelanotic melanoma cells suggests further storage compartments with quantitatively different contributions to the phototoxicity of bacteriochlorophyll-derived photosensitizers. Independent observations of early phototoxic effects on microfilamentous networks, enzymatic activities (succinate dehydrogenase, lactate dehydrogenase), and redistribution phenomena following primary uptake of the sensitizers let us assume that only a part of the 10⁸ molecules taken up by a cell contribute directly to phototoxicity. Thus it may be asked if a proper subcellular positioning of only a few sensitizer molecules may have similar phototoxic effects as the huge amounts stored at apparently ineffective sites.

Citation Download Citation

Joerg G. Moser, U. Dembeck, M. Hubert, Bernhard Spengler, Rainer Bayer, and Birgit Wagner "Subcellular storage compartments of bacteriopheophorbide sensitizers", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168704

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