Monte Carlo modeling of tissue autofluorescence measurement and imaging

Haishan Zeng; Calum E. MacAulay; Branko Palcic; David I. McLean M.D.

doi:10.1117/12.175985

19 May 1994 Monte Carlo modeling of tissue autofluorescence measurement and imaging

Haishan Zeng, Calum E. MacAulay, Branko Palcic, David I. McLean M.D.

Proceedings Volume 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases; (1994) https://doi.org/10.1117/12.175985
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

The major objective of this theoretical modeling was to examine the quantitative relationships between the microscopic properties of tissue and the macroscopic in vivo autofluorescence measurements. The modeling was carried out using skin tissue as an example. Monte Carlo simulation was used to model the excitation laser light distribution in and the autofluorescence escape process from skin tissue. The processes of fluorescence escape was modeled as the escape process from an isotropic point source embedded inside the tissue. The fractional contributions of different skin layers to the measured total in vivo autofluorescence signal were calculated and demonstrate good agreements with the results estimated from our laser induced autofluorescence decay experiments. This modeling also yielded the spatial resolution resolvable using tissue autofluorescence imaging. It was found to be worse than diffraction effect limited spatial resolution (equals the wavelength of light) and became significantly degraded for fluorescence sources deep inside the tissue.

Citation Download Citation

Haishan Zeng, Calum E. MacAulay, Branko Palcic, and David I. McLean M.D. "Monte Carlo modeling of tissue autofluorescence measurement and imaging", Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); https://doi.org/10.1117/12.175985

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available