Photodynamic decontamination of blood for transfusion

Ehud Ben-Hur; H. Margolis-Nunno; P. Gottlieb; S. Lustigman; Bernard Horowitz

doi:10.1117/12.199140

12 January 1995 Photodynamic decontamination of blood for transfusion

Ehud Ben-Hur, H. Margolis-Nunno, P. Gottlieb, S. Lustigman, Bernard Horowitz

Proceedings Volume 2325, Photodynamic Therapy of Cancer II; (1995) https://doi.org/10.1117/12.199140
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Currently transfused cellular components of blood are not available in a sterile form and carry a small risk of transmitting viral and parasite diseases. Using phthalocyanines and red light, lipid enveloped viruses, e.g., HIV-1, can be inactivated in red blood cell concentrates (RBCC). Under conditions leading to virus sterilization the blood borne parasites Trypanosoma cruzi (Chagas disease) and Plasmodium falciparum (malaria) could be eliminated to undetectable levels (> 4 log₁₀ kill). RBC damage during treatment could be avoided by increasing the light fluence rate to 80 mW/cm², and by including the free radical scavenger glutathione and the vitamin E derivative Trolox during light exposure. Similar sterilization of platelet concentrates was achieved with the psoralen derivative AMT and UVA light. Platelet damage due to PUVA treatment was avoided by including the plant flavonoid rutin during irradiation. It is concluded that elimination of the risk of transmitting pathogens during blood transfusion is feasible with photochemical treatments.

Citation Download Citation

Ehud Ben-Hur, H. Margolis-Nunno, P. Gottlieb, S. Lustigman, and Bernard Horowitz "Photodynamic decontamination of blood for transfusion", Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); https://doi.org/10.1117/12.199140

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