Light scattering by blood cells

Elena V. Konst; Vladimir B. Konstantinov; Lidia A. Bibikova

doi:10.1117/12.200877

1 February 1995 Light scattering by blood cells

Elena V. Konst, Vladimir B. Konstantinov, Lidia A. Bibikova

Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200877
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

There are many methods of testing blood parameters. The light scattered by blood cells forms the diffraction picture which contains all the information about number, size, and form of blood cells. Measuring the parameters of the spatial spectrum gives one the possibility to get the necessary data. To record the spectrum a computer aided CCD-camera is used. The scattering spectrum can be observed as a process of interference of beams refracted and reflected on the blood cell surfaces, interference of waves scattered by cells themselves many times. A typical spectrum looks like a system of bright and dark circles. The total area under the curve plotted from scanning the spectrum gives us an idea of the number of blood cells on the illuminated area of preparation. The diameter of first and following circles gives us information about size distribution of the cells. We have compared the spectrum of the blood preparations of healthy people and patients who passed through stress and shock. We propose that this method can be one of the quickest and easiest methods of blood-cell analysis.

Citation Download Citation

Elena V. Konst, Vladimir B. Konstantinov, and Lidia A. Bibikova "Light scattering by blood cells", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); https://doi.org/10.1117/12.200877

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