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1 March 1995 Photodynamic response of an endothelial hybridoma cell line using zinc(II) tetrasubstituted phthalocyanines
Janet E. Cruse-Sawyer, B. Dixon, David J.H. Roberts, John Griffiths, Stanley B. Brown
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Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203449
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States
Abstract
The EAhy 926 cell is a hybridoma line derived from human endothelium and A549/8 cells. They display stable endothelial characteristics and may provide an indication of how endothelial cells respond to photodynamic therapy. Cells were grown as monolayers, seeded at a density of 104 cells/35 mm dish, and then incubated with zinc (II) tetrasubstituted phthalocyanines (carboxylated, sulphonated, pyridinium or diethanolamine sulphonamide). After 24 hours, the cells were illuminated with laser light at 680 nm or 692 nm as appropriate. The response to each photosensitizer was evaluated using cell proliferation, clonogenicity, and release of tissue factor.
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Janet E. Cruse-Sawyer, B. Dixon, David J.H. Roberts, John Griffiths, and Stanley B. Brown "Photodynamic response of an endothelial hybridoma cell line using zinc(II) tetrasubstituted phthalocyanines", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203449
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KEYWORDS
Photodynamic therapy
Tissue optics
Zinc
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