X-ray microtomography: three-dimensional reconstruction methods for x-ray microscopy of biological samples

Billy W. Loo Jr.; J. Keenan Brown; Stephen S. Rothman

doi:10.1117/12.205339

23 March 1995 X-ray microtomography: three-dimensional reconstruction methods for x-ray microscopy of biological samples

Billy W. Loo Jr., J. Keenan Brown, Stephen S. Rothman

Author Affiliations +

Proceedings Volume 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II; (1995) https://doi.org/10.1117/12.205339
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1995, San Jose, CA, United States

Abstract

Biological soft x-ray microscopy using x-ray optics and synchrotron sources has made possible quantitative, element-specific imaging of whole cells in aqueous media at significantly higher resolutions than those of conventional visible light methods. Tomographic reconstruction has been proposed as a means to realize the full potential of the method for viewing thick objects whose structures would otherwise be superimposed in single view projections. The authors present an iterative tomographic reconstruction algorithm, using a regularized weighted least squares objective function, accelerated with the conjugate gradient approach, and modified for the problem of transmission tomography with correction of blurring by an instrumental point spread function. The non-negativity constraint is implemented using a preconditioner. We show by computer simulations that reconstructions that meet realistic and acceptable goals for spatial and density resolution should be achievable at doses compatible with the structural integrity of biological samples at the specific resolution.

Citation Download Citation

Billy W. Loo Jr., J. Keenan Brown, and Stephen S. Rothman "X-ray microtomography: three-dimensional reconstruction methods for x-ray microscopy of biological samples", Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); https://doi.org/10.1117/12.205339

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