Tumor diagnostics using fiber optical IR spectroscopy

Harald Winter; Uwe Bindig; Wolfgang Waesche; K. Liebold; Andre Roggan; P. Frege; Ulrich M. Gross; G. Mueller

doi:10.1117/12.346716

21 April 1999 Tumor diagnostics using fiber optical IR spectroscopy

Harald Winter, Uwe Bindig, Wolfgang Waesche, K. Liebold, Andre Roggan, P. Frege, Ulrich M. Gross, G. Mueller

Proceedings Volume 3596, Specialty Fiber Optics for Medical Applications; (1999) https://doi.org/10.1117/12.346716
Event: BiOS '99 International Biomedical Optics Symposium, 1999, San Jose, CA, United States

Abstract

Aim of the project is the development of an in vivo endoscopic method to differentiate between cancerous from healthy tissue. The method is based on IR spectra in which each diseased state of the tissue has its own characteristic pattern as already shown in previous experiments. Two regions (1245 - 1195) cm^-1 and (1045 - 995) cm^-1 within the fingerprint (less than 1500 cm^-1) region were selected for analysis. This paper will present the technical design of the laboratory set up and outcome of the development as well as the experiments. Two lead-salt diode lasers were used as excitation sources. The IR-radiation was transmitted via silverhalide fibers to the tissue to be investigated. On the detection side another IR fiber was used to transmit the signal to an MCT-detector (Mercury-Cadmium-Telluride). Measurement modes are Attenuated Total Reflectance (ATR) and diffuse Reflection/Remission. Spatial resolution was 100 X 100 micrometer². The tissue used for these experiments was human colon carcinoma under humidity conditions. Samples were mapped using a stepper motor powered x/y/z-translation stage with a resolution of 1 micrometer. Results were compared with measurements carried out using a FTIR-interferometer and an FTIR-microscope in the region from 4000 - 900 cm^-1. Soft- and Hardware control of the experiment is done using Labwindows/CVI (National Instruments, USA).

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Harald Winter, Uwe Bindig, Wolfgang Waesche, K. Liebold, Andre Roggan, P. Frege, Ulrich M. Gross, and G. Mueller "Tumor diagnostics using fiber optical IR spectroscopy", Proc. SPIE 3596, Specialty Fiber Optics for Medical Applications, (21 April 1999); https://doi.org/10.1117/12.346716

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KEYWORDS

Tissues

Diodes

Semiconductor lasers

Microscopes

Tumors

Tissue optics

Colon

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