Differential phase contrast OCT in transparant and scattering media

Markus Sticker; Christoph K. Hitzenberger; Rainer Leitgeb; Adolf Friedrich Fercher

doi:10.1117/12.427906

23 May 2001 Differential phase contrast OCT in transparant and scattering media

Markus Sticker, Christoph K. Hitzenberger, Rainer Leitgeb, Adolf Friedrich Fercher

Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427906
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Many biological objects have a poor contrast in microscopy when they are imaged on the basis of the intensity of transmitted and reflected light. For pure phase objects the differential phase contrast technique increases the contrast of the images. We combined the differential phase contrast technique with optical coherence tomography. Our setup is based on a Michelson interferometer with a polarization sensitive detection unit. We scan the sample with two orthogonally polarized beams, which are separated by a distance of 17.5 micrometers . The full interferometric signal of each object beam is recorded by a separate detector. We calculate the phase functions of the interferometric signal through analytic continuation by use of the Hilbert transformation. Subtracting the two phase functions we get the phase difference between the object beams. Now we can derive the path length difference of the object beams at a certain depth in the object where the light was backscattered. The method is independent of variations in the backscattering coefficient, which was a problem in an earlier version of our setup. To investigate the performance of the technique we measured pure phase objects in the nm range. Differential phase measurements through scattering test samples quantified the influence of scattering on the phase measurement. First images of cell structures are presented.

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Markus Sticker, Christoph K. Hitzenberger, Rainer Leitgeb, and Adolf Friedrich Fercher "Differential phase contrast OCT in transparant and scattering media", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427906

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KEYWORDS

Optical coherence tomography

Phase contrast

Scattering

Beam splitters

Polarization

Phase measurement

Microscopy

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