Characterization of the second harmonic signal from collagen

Guy C. Cox; Paul Xu; Colin J. R. Sheppard; John A. Ramshaw

doi:10.1117/12.485598

10 July 2003 Characterization of the second harmonic signal from collagen

Guy C. Cox, Paul Xu, Colin J. R. Sheppard, John A. Ramshaw

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.485598
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Collagen is known to be a very effective generator of the second harmonic of incident light from 700 to 1100nm, and second harmonic generation (SHG) microscopy is coming into use as a tool for studying the distribution of collagen in tissue. It also shows promise as a technique for characterizing collagen - both in distinguishing different collagen types and their packing and in identifying degradation of collagen in pathologic conditions. However many aspects of image formation in SHG microscopy of collagen remain imperfectly understood, and we have commenced a rigorous study of these factors. The present paper presents the first results from this program.

Citation Download Citation

Guy C. Cox, Paul Xu, Colin J. R. Sheppard, and John A. Ramshaw "Characterization of the second harmonic signal from collagen", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.485598

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