Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs

Chih-kuan Tung; Tsung-Kai Chiu; Wen Lo; Po-Hsiang Wang; Sun-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.478026

10 July 2003 Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs

Chih-kuan Tung, Tsung-Kai Chiu, Wen Lo, Po-Hsiang Wang, Sun-Jan Lin, Shiou-Hwa Jee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478026
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Index mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples.

Citation Download Citation

Chih-kuan Tung, Tsung-Kai Chiu, Wen Lo, Po-Hsiang Wang, Sun-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong "Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478026

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE