Fluorescence decay profile measured with spread spectrum excitations

Nan Guang Chen; Quing Zhu

doi:10.1117/12.478008

10 July 2003 Fluorescence decay profile measured with spread spectrum excitations

Nan Guang Chen, Quing Zhu

Proceedings Volume 4963, Multiphoton Microscopy in the Biomedical Sciences III; (2003) https://doi.org/10.1117/12.478008
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

A novel excitation and detection scheme has been proposed to measure time-resolved fluorescence. A train of continuous excitation pulses modulated by a pseudo-random bit sequence, instead of periodical short pulses of a very low duty factor, is used as the light source to illuminate a fluorescent sample. The cross-correlation between the emission from the sample and the modulation sequence yields the temporal profile of fluorescence decay. We have developed a primitive PC-based time-resolved fluorometer, in which an ultraviolet LED is modulated by a 4095-bit long maximal length pseudo-random bit sequence. The temporal resolution of the fluorometer is up to 1 microsecond. A long lifetime (more than 300 microseconds) europium chelate was used to test our instrument. Its fluorescence decay profile was successfully retrieved, and the measured lifetime is compatible with the specification.

Citation Download Citation

Nan Guang Chen and Quing Zhu "Fluorescence decay profile measured with spread spectrum excitations", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); https://doi.org/10.1117/12.478008

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