Investigation of doxorubicin for multidrug resistance using a fluorescent cytometric imaging system integrated onto cell culture analog devices

Donghyun Kim; Hui Xu; Sung June Kim; Michael L. Shuler

doi:10.1117/12.523626

18 June 2004 Investigation of doxorubicin for multidrug resistance using a fluorescent cytometric imaging system integrated onto cell culture analog devices

Donghyun Kim, Hui Xu, Sung June Kim, Michael L. Shuler

Author Affiliations +

Proceedings Volume 5325, Optical Diagnostics and Sensing IV; (2004) https://doi.org/10.1117/12.523626
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

An integrated cytometric fluorescent imaging system is developed for characterizing chemical concentration and cellular status in microscale cell culture analog (μCCA) devices. A μCCA is used to evaluate the potential toxicity and efficacy of proposed pharmaceutical treatment of animals or humans. The imaging system, based on discrete optical components, not only provides a robust and compact tool for real-time measurements, but the modularity of the system also offers flexibility to be applicable to various μCCA structures that may be appropriate to various animal or human models. We investigate the dynamics of doxorubicin, a chemotherapeutic agent, on cultured cells in a μCCA using the integrated cytometric fluorescent imaging system. This study incorporates two uteran cancer cell lines representing a sensitive cell type and a multi-drug resistant (MDR) derivative cell line. The ultimate goal is to test the effect of MDR modulators in combination with doxorubicin to kill cancer cells while not causing undue harm to normal cells.

Citation Download Citation

Donghyun Kim, Hui Xu, Sung June Kim, and Michael L. Shuler "Investigation of doxorubicin for multidrug resistance using a fluorescent cytometric imaging system integrated onto cell culture analog devices", Proc. SPIE 5325, Optical Diagnostics and Sensing IV, (18 June 2004); https://doi.org/10.1117/12.523626

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