Multiphoton tomography, transfection, and nanosurgery with <2-nJ, 80-MHz femtosecond laser pulses

Karsten Koenig

doi:10.1117/12.528240

1 June 2004 Multiphoton tomography, transfection, and nanosurgery with <2-nJ, 80-MHz femtosecond laser pulses

Karsten Koenig

Proceedings Volume 5340, Commercial and Biomedical Applications of Ultrafast Lasers IV; (2004) https://doi.org/10.1117/12.528240
Event: Lasers and Applications in Science and Engineering, 2004, San Jose, Ca, United States

Abstract

Biomedical applications of low-energy (< 2nJ) near infrared (NIR) femtosecond laser pulses provided by compact, turn-key Ti:sapphire lasers are presented in this review. Applications include (i) ultrahigh resolution optical diagnostics ("optical biopsies"), (ii) gene therapy by optical targeted transfection of cells, and (iii) ultraprecise laser therapy ("nanosurgery"). The novel femtosecond laser system DermaInspec (JenLab GmbH) enables for the first time in vivo deep tissue imaging of intracellular compartments with submicron spatial and picosecond temporal resolution in patients with dermatological disorders. Using the system FemtOcut, intracellular surgery, optical gene transfer, and intraocular refractive surgery can be performed. The major process behind the diagnostical and therapeutical laser effects is non-resonant multiphoton absorption which results in two-photon autofluorescence and second harmonic generation at transient intensities of GW/cm² as well as multiphoton ionization and plasma formation at TW/cm² intensities, respectively.

Citation Download Citation

Karsten Koenig "Multiphoton tomography, transfection, and nanosurgery with <2-nJ, 80-MHz femtosecond laser pulses", Proc. SPIE 5340, Commercial and Biomedical Applications of Ultrafast Lasers IV, (1 June 2004); https://doi.org/10.1117/12.528240

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