Subwavelength-resolution fluorescent correlation spectroscopy using a solid immersion lens

Alexandre Serov; Ramachandra G. Rao; Michael Gosch; Tiemo Anhut; Dietrich Martin; Robert Brunner; Rudolf Rigler; Theo Lasser

doi:10.1117/12.572046

29 July 2004 Subwavelength-resolution fluorescent correlation spectroscopy using a solid immersion lens

Alexandre Serov, Ramachandra G. Rao, Michael Gosch, Tiemo Anhut, Dietrich Martin, Robert Brunner, Rudolf Rigler, Theo Lasser

Author Affiliations +

Proceedings Volume 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics; (2004) https://doi.org/10.1117/12.572046
Event: ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, 2003, Silsoe, United Kingdom

Abstract

In this paper we present recent spectroscopic studies using a Solid Immersion Lens for Fluorescent Correlation Spectroscopy measurements. We compare the performance of the Solid Immersion Lens confocal microscope built-up in our group to the performance of a conventional confocal microscope used for FCS. The novelty of the new SIL-FCS microscope is a system containing a conventional objective (NA = 0.6) combined with a Solid Immersion Lens used for single molecule experiment. Important parameters for single molecule experiments such as collection efficiency and excitation field confinement are investigated for different modes of the SIL objective system.

Citation Download Citation

Alexandre Serov, Ramachandra G. Rao, Michael Gosch, Tiemo Anhut, Dietrich Martin, Robert Brunner, Rudolf Rigler, and Theo Lasser "Subwavelength-resolution fluorescent correlation spectroscopy using a solid immersion lens", Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); https://doi.org/10.1117/12.572046

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available