Multiple UV wavelength excitation and fluorescence of bioaerosols

Vasanthi Sivaprakasam; Alan L. Huston; Cathy S. Scotto; Jay D. Eversole

doi:10.1117/12.571296

16 December 2004 Multiple UV wavelength excitation and fluorescence of bioaerosols

Vasanthi Sivaprakasam, Alan L. Huston, Cathy S. Scotto, Jay D. Eversole

Proceedings Volume 5585, Chemical and Biological Point Sensors for Homeland Defense II; (2004) https://doi.org/10.1117/12.571296
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

A two-wavelength excitation bioaerosol sensor has been developed and characterized for classifying various types of aerosols, including biological organisms and non-biological interferents. Single aerosols, smaller than 10 μm, are interrogated with 266 nm and 355 nm laser pulses separated in time by 400 ns. Fluorescence signals excited by these pulses are detected in three broad spectral bands centered at 350 nm, 450 nm and 550 nm. The results indicate that bacterial spores, vegetative bacterial cells and proteins can be differentiated based on the two wavelength excitation approach.

Citation Download Citation

Vasanthi Sivaprakasam, Alan L. Huston, Cathy S. Scotto, and Jay D. Eversole "Multiple UV wavelength excitation and fluorescence of bioaerosols", Proc. SPIE 5585, Chemical and Biological Point Sensors for Homeland Defense II, (16 December 2004); https://doi.org/10.1117/12.571296

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