Laser speckle contrast imaging: monitoring blood flow dynamics and vascular structure of photodynamic therapy

Qian Liu D.V.M.; Sibo Zhou; Zhihong Zhang; Qingming Luo

doi:10.1117/12.572202

18 January 2005 Laser speckle contrast imaging: monitoring blood flow dynamics and vascular structure of photodynamic therapy

Qian Liu D.V.M., Sibo Zhou, Zhihong Zhang, Qingming Luo

Author Affiliations +

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.572202
Event: Photonics Asia, 2004, Beijing, China

Abstract

Laser speckle contrast imaging (LSCI) is a noninvasive optical image technique that has been developed for imaging in vivo blood flow dynamics and vascular structure with high spatial and temporal resolution. It records the full-field spatio-temporal characteristics of microcirculation in real time without the need of laser beam flying. In this paper applications of this technique for monitoring changes of blood flow and vascular structure following photodynamic therapy (PDT) in vivo model were demonstrated. In this study, an in vivo model of chick chorioallantoic membrane (CAM) at embryo age (EA) of 10~13 days, was observed following PDT irradiated by a power tunable laser diode (λ = 656.5 nm). Laser intensity incident on the treatment site was maintained at 40 mW/cm² and photosensitizer of Pyropheophorbide Acid (Pyro-Acid) was used. CAM was adopted in PDT since it is a transparent in vivo model and the irradiated lights of laser can penetrate tumor with greater depth. The laser delivered through fiber bundle to the treatment site in PDT also acted as the coherent light source of LSCI. This study shows that LSCI can be used to assess the efficacy of peripheral vessels damage of tumor in PDT by monitoring changes of blood flow and vascular structure.

Citation Download Citation

Qian Liu D.V.M., Sibo Zhou, Zhihong Zhang, and Qingming Luo "Laser speckle contrast imaging: monitoring blood flow dynamics and vascular structure of photodynamic therapy", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.572202

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