Two-photon and second harmonic generation microscopy of porcine eye: implications to conductive keratoplasty

Shu-Wen Teng; Ju-Li Peng; Huei-Hsing Lin; Hai-Yin Wu; Hsin-Yuan Tan; Wen Lo; Wei-Chou Lin; Sung-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.597043

30 March 2005 Two-photon and second harmonic generation microscopy of porcine eye: implications to conductive keratoplasty

Shu-Wen Teng, Ju-Li Peng, Huei-Hsing Lin, Hai-Yin Wu, Hsin-Yuan Tan, Wen Lo, Wei-Chou Lin, Sung-Jan Lin, Shiou-Hwa Jee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.597043
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Two-photon autofluorescence and second harmonic generation (SHG) microscopy are useful imaging techniques for studying tissue components. In this work, we apply this imaging modality to study porcine eye. In particular, we use SHG microscopy to investigate the structural changes to excised porcine corneas and found that this technique is useful for studying its structural changes under thermal treatment.

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Shu-Wen Teng, Ju-Li Peng, Huei-Hsing Lin, Hai-Yin Wu, Hsin-Yuan Tan, Wen Lo, Wei-Chou Lin, Sung-Jan Lin, Shiou-Hwa Jee, and Chen-Yuan Dong "Two-photon and second harmonic generation microscopy of porcine eye: implications to conductive keratoplasty", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.597043

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