The effects of self-absorption and detection geometry on fluorescence intensity and decay lifetime

Jean-Francois Cormier; Michel Fortin; Julie Frechette; Isabelle Noiseux; Marcia L. Vernon; William Long

doi:10.1117/12.591026

7 March 2005 The effects of self-absorption and detection geometry on fluorescence intensity and decay lifetime

Jean-Francois Cormier, Michel Fortin, Julie Frechette, Isabelle Noiseux, Marcia L. Vernon, William Long

Proceedings Volume 5702, Optical Diagnostics and Sensing V; (2005) https://doi.org/10.1117/12.591026
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

This paper presents a theoretical model of the effect of the geometry of illumination and collection in fluorescent media, which exhibit self-absorption at sufficiently high concentrations. In order to derive a relation between the incident excitation intensity and the fluorescence emission intensity, we consider the series of paths and transformations that light takes between the source and the detector. The preliminary supporting experiments were conducted on non-turbid liquid fluorescent samples using classical right-angle detection scheme, based on Time-Correlated Single Photon Counting (TCSPC). The fluorescent dyes tested in these experiments (Coumarins 1, 314 and 343) were chosen because they all are excitable at 405 nm, and exhibit varying Stokes shifts. The results suggest that the geometry of the illumination and collection, as well as the self-absorption process, should be taken into account in time-resolved and intensity fluorescence measurements.

Citation Download Citation

Jean-Francois Cormier, Michel Fortin, Julie Frechette, Isabelle Noiseux, Marcia L. Vernon, and William Long "The effects of self-absorption and detection geometry on fluorescence intensity and decay lifetime", Proc. SPIE 5702, Optical Diagnostics and Sensing V, (7 March 2005); https://doi.org/10.1117/12.591026

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available