The LSM 5 family: an integrated imaging and spectroscopic platform for the study of cellular dynamic processes

Klaus Weisshart

doi:10.1117/12.646299

23 February 2006 The LSM 5 family: an integrated imaging and spectroscopic platform for the study of cellular dynamic processes

Klaus Weisshart

Author Affiliations +

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 608909 (2006) https://doi.org/10.1117/12.646299
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

The elucidation of diffusion processes and molecular interactions and their relation to compartments and structures will be essential to understand cellular functions in detail. Often it is not the average signal that is of interest but the behaviour of single molecules which behave as individuals. Fluorescence based assays have revolutionized the way we can observe molecules at work in their natural cellular settings and they have now also become available for single molecule studies. These technologies comprise Fluorescence Fluctuation Analysis (FFA) including Fluorescence Correlation Spectroscopy (FCS), Fluorescence Redistribution After Photobleaching (FRAP), Foerster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM). Especially for dual colour experiments and when dealing with delicate samples the employment of multiphoton microscopy using the aforementioned technologies can be of great benefit. Ideal instruments to study single molecules would therefore need to accommodate equipment that allow for fast time resolution, adequate detectors and lasers as well as integrated work flows. In this contribution we discuss the newest developments in commercial instrumentation and software at Carl Zeiss towards highly sensitive imaging in combination with spectroscopic analysis.

Citation Download Citation

Klaus Weisshart "The LSM 5 family: an integrated imaging and spectroscopic platform for the study of cellular dynamic processes", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 608909 (23 February 2006); https://doi.org/10.1117/12.646299

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Molecules

Proteins

Fluorescence correlation spectroscopy

Fluorescence resonance energy transfer

Luminescence

Image acquisition

Diffusion

Show All Keywords

Keywords/Phrases

Search In:

Publication Years