Highly sensitive single photon detection system for multilane DNA sequencer

Dmitri Gavrilov; Boris Gorbovitski; Georgiy Gudkov; Andrey Stepukhovich; Marina Tcherevishnik; Georgiy Tyshko; Andriy Tsupryk; Vera Gorfinkel

doi:10.1117/12.685404

3 November 2006 Highly sensitive single photon detection system for multi-lane DNA sequencer

Dmitri Gavrilov, Boris Gorbovitski, Georgiy Gudkov, Andrey Stepukhovich, Marina Tcherevishnik, Georgiy Tyshko, Andriy Tsupryk, Vera Gorfinkel

Author Affiliations +

Proceedings Volume 6372, Advanced Photon Counting Techniques; 63720B (2006) https://doi.org/10.1117/12.685404
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

A multichannel fluorescence detection system for multi-lane DNA sequencer based on single photon counting is presented. The problem of elimination of the lane cross-talk caused by both optical and electronic cross-talk between the detection lanes is discussed. A novel method for elimination of the channel cross-talk is proposed. The calibration procedure for a DNA sequencer with lane cross-talk cancellation is described. Improvement in sensitivity of a DNA sequencer setup by introducing wide collection angle optical system is discussed.

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Dmitri Gavrilov, Boris Gorbovitski, Georgiy Gudkov, Andrey Stepukhovich, Marina Tcherevishnik, Georgiy Tyshko, Andriy Tsupryk, and Vera Gorfinkel "Highly sensitive single photon detection system for multi-lane DNA sequencer", Proc. SPIE 6372, Advanced Photon Counting Techniques, 63720B (3 November 2006); https://doi.org/10.1117/12.685404

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