Wide-field photon counting imaging for fluorescence microscopy

Nicolas Sergent; James A. Levitt; Mark A. Green; Klaus Suhling

doi:10.1117/12.733793

15 October 2007 Wide-field photon counting imaging for fluorescence microscopy

Nicolas Sergent, James A. Levitt, Mark A. Green, Klaus Suhling

Proceedings Volume 6771, Advanced Photon Counting Techniques II; 67710X (2007) https://doi.org/10.1117/12.733793
Event: Optics East, 2007, Boston, MA, United States

Abstract

We present a novel time-resolved photon counting imaging technique and its use in multi-dimensional luminescence spectroscopy. By using an ultrafast camera coupled to an image intensifier on a microscope, we demonstrate the potential of wide-field time-correlated single photon counting, with a count rate of up to 5 Mhz. This system has the advantage of allowing the detection of single photons in parallel in every pixel. We measured the luminescence decay of Europium Polyoxometalate (POM), and observed contrast on lifetime images of Eu-POM on silver nanocrystals.

Citation Download Citation

Nicolas Sergent, James A. Levitt, Mark A. Green, and Klaus Suhling "Wide-field photon counting imaging for fluorescence microscopy", Proc. SPIE 6771, Advanced Photon Counting Techniques II, 67710X (15 October 2007); https://doi.org/10.1117/12.733793

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