QSIP: phase imaging made possible in a bright field microscope

Sri Rama Prasanna Pavani; Ariel R. Libertun; Sharon V. King; Carol J. Cogswell

doi:10.1117/12.762131

12 February 2008 QSIP: phase imaging made possible in a bright field microscope

Sri Rama Prasanna Pavani, Ariel R. Libertun, Sharon V. King, Carol J. Cogswell

Proceedings Volume 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV; 686105 (2008) https://doi.org/10.1117/12.762131
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Quantitative structured-illumination phase microscopy (QSIP) uses a conventional bright field microscope to quantitatively measure the optical path length profiles of homogenous phase-only objects. The illumination in QSIP is structured with a predetermined pattern by placing an amplitude mask in the field diaphragm of the microscope. From the image of the amplitude mask, a numerical algorithm implementing a closed form analytical solution calculates the object's optical path length profile. In this paper, we investigate the accuracy of the numerical algorithm used and show that it can be made arbitrarily accurate by using numerical optimization. We then analyze the effect of the system's numerical aperture (NA), and show that QSIP can be used with a wide range of NAs for objects with small phase gradients, and can be used with relatively lower NAs for objects with large phase gradients.

Citation Download Citation

Sri Rama Prasanna Pavani, Ariel R. Libertun, Sharon V. King, and Carol J. Cogswell "QSIP: phase imaging made possible in a bright field microscope", Proc. SPIE 6861, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XV, 686105 (12 February 2008); https://doi.org/10.1117/12.762131

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