Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device

Brian C. Heinze; Jae-Young Song D.V.M.; Jin-Hee Han; Jeong-Yeol Yoon

doi:10.1117/12.761341

1 March 2008 Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device

Brian C. Heinze, Jae-Young Song D.V.M., Jin-Hee Han, Jeong-Yeol Yoon

Author Affiliations +

Proceedings Volume 6886, Microfluidics, BioMEMS, and Medical Microsystems VI; 688605 (2008) https://doi.org/10.1117/12.761341
Event: MOEMS-MEMS 2008 Micro and Nanofabrication, 2008, San Jose, California, United States

Abstract

We have investigated the utilization of particle agglutination assays using forward light scattering measurements in a microfluidic device towards detecting viral particles. The model viral target was bovine viral diarrhea virus (BVDV). Highly carboxylated polystyrene microspheres (510 nm) were coated with anti-BVDV monoclonal antibodies. This solution was in turn used to detect live modified BVDV. This assay was first performed in a two well slide for proof of concept and then in a simple y-channel microfluidic device with optical fibers arranged in a close proximity setup. Particle immunoagglutination was detected through static light scattering measurements taken at 45° to incident light. In the microfluidic device, modified live BVDV was detected with a detection limit of 0.5 TCID₅₀ mL^-1.

Citation Download Citation

Brian C. Heinze, Jae-Young Song D.V.M., Jin-Hee Han, and Jeong-Yeol Yoon "Latex immunoagglutination assay for bovine viral diarrhea virus utilizing forward light scattering in a microfluidic device", Proc. SPIE 6886, Microfluidics, BioMEMS, and Medical Microsystems VI, 688605 (1 March 2008); https://doi.org/10.1117/12.761341

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