Combining confocal microscopy and optical coherence tomography for imaging in developmental biology

A. Bradu; Lisha Ma; J. Bloor; A. Podoleanu

doi:10.1117/12.781041

2 May 2008 Combining confocal microscopy and optical coherence tomography for imaging in developmental biology

A. Bradu, Lisha Ma, J. Bloor, A. Podoleanu

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69910K (2008) https://doi.org/10.1117/12.781041
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

In-vivo Optical Coherence Tomography (OCT) imaging of the fruit fly Drosophila melanogaster larval heart allows non invasive visualizations and assesment of its cardiac functions. To image Drosophila melanogaster heart, we have developed a dedicated imaging instrument able to provide simultaneous Optical Coherence Tomography (OCT) and Laser Confocal Scanning Microscopy (LCSM) or Laser Scanning Fluorescence Microscopy (LSFM) images and can be used to produce B-scan OCT images. With this dual imaging system, the image of heart can be easily located in the specimen and the change of the heart shape in a cardiac cycle monitored. This technique therefore provides an excellent tool for large scale screen of candidate genes responsible for the contractility of the Drosophila heart. As this technique can also image the dynamic process of the heartbeat in a non-invasive fashion, it provides a new avenue to study the physiology of the heart function. En-face and B-scan OCT images of the Drosophila melanogaster heart showing its chambers have been obtained with our imaging instruments. Our results are consistent with detailed anatomical studies from the literature.

Citation Download Citation

A. Bradu, Lisha Ma, J. Bloor, and A. Podoleanu "Combining confocal microscopy and optical coherence tomography for imaging in developmental biology", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69910K (2 May 2008); https://doi.org/10.1117/12.781041

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