Monitoring blood flow and photobleaching during topical ALA PDT treatment

Theresa L. Sands; Ulas Sunar; Thomas H. Foster; Allan R. Oseroff M.D.

doi:10.1117/12.809217

18 February 2009 Monitoring blood flow and photobleaching during topical ALA PDT treatment

Theresa L. Sands, Ulas Sunar, Thomas H. Foster, Allan R. Oseroff M.D.

Proceedings Volume 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII; 71640U (2009) https://doi.org/10.1117/12.809217
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Photodynamic therapy (PDT) using topical aminolevulinic acid (ALA) is currently used as a clinical treatment for nonmelanoma skin cancers. In order to optimize PDT treatment, vascular shutdown early in treatment must be identified and prevented. This is especially important for topical ALA PDT where vascular shutdown is only temporary and is not a primary method of cell death. Shutdown in vasculature would limit the delivery of oxygen which is necessary for effective PDT treatment. Diffuse correlation spectroscopy (DCS) was used to monitor relative blood flow changes in Balb/C mice undergoing PDT at fluence rates of 10mW/cm² and 75mW/cm² for colon-26 tumors implanted intradermally. DCS is a preferable method to monitor the blood flow during PDT of lesions due to its ability to be used noninvasively throughout treatment, returning data from differing depths of tissue. Photobleaching of the photosensitizer was also monitored during treatment as an indirect manner of monitoring singlet oxygen production. In this paper, we show the conditions that cause vascular shutdown in our tumor model and its effects on the photobleaching rate.

Citation Download Citation

Theresa L. Sands, Ulas Sunar, Thomas H. Foster, and Allan R. Oseroff M.D. "Monitoring blood flow and photobleaching during topical ALA PDT treatment", Proc. SPIE 7164, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVIII, 71640U (18 February 2009); https://doi.org/10.1117/12.809217

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