Detection of methicillin-resistant Staphylococcus aureus (MRSA) using the NanoLantern Biosensor

Christopher M. Strohsahl; Benjamin L. Miller; Todd D. Krauss

doi:10.1117/12.808872

24 February 2009 Detection of methicillin-resistant Staphylococcus aureus (MRSA) using the NanoLantern Biosensor

Christopher M. Strohsahl, Benjamin L. Miller, Todd D. Krauss

Proceedings Volume 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems; 71670S (2009) https://doi.org/10.1117/12.808872
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Staphylococcus aureus is a leading cause of human illness, and has developed the remarkable ability to resist the bactericidal capabilities of many of the world's leading antibiotics (i.e. MRSA). In an effort to enable rapid detection and treatment of MRSA infections, we have developed a DNA detection technology termed the NanoLantern(TM). The NanoLantern(TM) biosensor technology is based on the simple immobilization of a fluorophore-terminated DNA hairpin onto a gold chip. This produces a label-free sensor that allows for a positive response to be obtained without extensive processing of the sample, saving cost and increasing accuracy. We will also discuss a newly developed method of partial gene analysis, used to develop a DNA hairpin probe that is capable of detecting the presence of the mecR gene, a gene necessary for methicillin resistance to be present in S. aureus, with 100% sequence specificity. The successful incorporation of this probe into the NanoLantern(TM) platform, along with the concomitant development of the paired PCR assay has allowed for the successful detection of methicillin-resistance directly from a culture of S. aureus. These results represent an important step forward in terms of developing the ability to rapidly and effectively detect the presence of antibiotic resistance in bacterial infections.

Citation Download Citation

Christopher M. Strohsahl, Benjamin L. Miller, and Todd D. Krauss "Detection of methicillin-resistant Staphylococcus aureus (MRSA) using the NanoLantern Biosensor", Proc. SPIE 7167, Frontiers in Pathogen Detection: From Nanosensors to Systems, 71670S (24 February 2009); https://doi.org/10.1117/12.808872

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