Multimodal plasmonic nanosensor for the detection of pathogenic bacteria

Li-Lin Tay; John Hulse; Shannon Ryan; Jamshid Tanha; Jeff Fraser; Xiaohua Wu

doi:10.1117/12.826093

25 August 2009 Multimodal plasmonic nanosensor for the detection of pathogenic bacteria

Li-Lin Tay, John Hulse, Shannon Ryan, Jamshid Tanha, Jeff Fraser, Xiaohua Wu

Proceedings Volume 7397, Biosensing II; 73970B (2009) https://doi.org/10.1117/12.826093
Event: SPIE NanoScience + Engineering, 2009, San Diego, California, United States

Abstract

Multi-modal sensing scheme significantly improves the detection accuracy but can also introduce extra complexity in the overall design of the sensor. We overcome this difficulty by utilizing the plasmonic properties of metallic nanoparticles. In this study, we will present a simple dual optical sensing mechanism which harvests signals of the resonantly excited metallic nanostructure in the form of surface enhanced Raman scattering (SERS) and resonant Rayleigh scattering. Silver and gold nanoparticles labeled with appropriate antibodies act as signal transduction units and upon exposure to the targeted pathogen render the targeted species optically active. We demonstrate that detection of a single pathogen cell is easily attainable with the dual detection scheme. Furthermore, we explore the markedly different SERS intensity observed from the use of two very different antibody recognition units during the pathogen labeling process.

Citation Download Citation

Li-Lin Tay, John Hulse, Shannon Ryan, Jamshid Tanha, Jeff Fraser, and Xiaohua Wu "Multimodal plasmonic nanosensor for the detection of pathogenic bacteria", Proc. SPIE 7397, Biosensing II, 73970B (25 August 2009); https://doi.org/10.1117/12.826093

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