Studies on molecular recognition of thymidines with molecularly imprinted polymers

Zhen-He Chen; Ai-Qin Luo; Li-Quan Sun

doi:10.1117/12.847732

20 October 2009 Studies on molecular recognition of thymidines with molecularly imprinted polymers

Zhen-He Chen, Ai-Qin Luo, Li-Quan Sun

Proceedings Volume 7493, Second International Conference on Smart Materials and Nanotechnology in Engineering; 74931Y (2009) https://doi.org/10.1117/12.847732
Event: Second International Conference on Smart Materials and Nanotechnology in Engineering, 2009, Weihai, China

Abstract

Molecularly imprinted polymers (MIPs) with excellent molecular recognition ability have been used in chemical sensors, chromatographic separation and biochemical analyses. Thymidine is an important part of DNA for biomolecular recognition and the intermediate of many medicines. The polymers imprinted with the template of thymidine and 5'-Otosylthymidine have been prepared, using a non-proton solvent, acetonitrile as the porogen. Direct imprinting with thymidine could not form strong molecular interaction sites in this system. Relative MIPs were obtained by bulk polymerization and their adsorption capacities were investigated. The adsorption capacities of MIP (P₂) and nonimprinted polymer (P₂₀) for thymidine are 0.120 mg•g^-1and 0.103 mg•g^-1, respectively. The imprinting factor is 1.17. As 5'-O-tosylthymidine is more soluble than thymidine moiety in acetonitrile and give rise to more sites of molecular recognition. The results demonstrated that the imprinted polymers were able to bind and recognize thymidine moderately in acetonitrile. MIPs imprinted with 5'-O-tosylthymidine like nature enzymes displayed some recognition ability to its analogues. The insoluble derivatives in the non-proton solvent can be an effective template to prepare efficient imprinting recognition sites.

Citation Download Citation

Zhen-He Chen, Ai-Qin Luo, and Li-Quan Sun "Studies on molecular recognition of thymidines with molecularly imprinted polymers", Proc. SPIE 7493, Second International Conference on Smart Materials and Nanotechnology in Engineering, 74931Y (20 October 2009); https://doi.org/10.1117/12.847732

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