Vibrational phase contrast CARS microscopy for quantitative analysis

M. Jurna; E. T. Garbacik; J. P. Korterik; C. Otto; J. L. Herek; H. L. Offerhaus

doi:10.1117/12.840178

26 February 2010 Vibrational phase contrast CARS microscopy for quantitative analysis

M. Jurna, E. T. Garbacik, J. P. Korterik, C. Otto, J. L. Herek, H. L. Offerhaus

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75690F (2010) https://doi.org/10.1117/12.840178
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In biological samples the resonant CARS signal of less abundant constituents can be overwhelmed by the nonresonant background, preventing detection of those molecules. We demonstrate a method to obtain the phase of the oscillators in the focal volume that allows discrimination of those hidden molecules. The phase is measured with respect to the local excitation fields using a cascaded phase-preserving chain. It is measured point-bypoint and takes into account refractive index changes in the sample, phase curvature over the field-of-view and interferometric instabilities. The detection of the phase of the vibrational motion can be regarded as a vibrational extension of the linear (refractive index) phase contrast microscopy introduced by Zernike around 1933.

Citation Download Citation

M. Jurna, E. T. Garbacik, J. P. Korterik, C. Otto, J. L. Herek, and H. L. Offerhaus "Vibrational phase contrast CARS microscopy for quantitative analysis", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690F (26 February 2010); https://doi.org/10.1117/12.840178

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