In situ observation of collagen thermal denaturation by second harmonic generation microscopy

C.-S. Liao; Z.-Y. Zhuo; J.-Y. Yu; P.-H. G. Chao; S.-W. Chu

doi:10.1117/12.841204

26 February 2010 In situ observation of collagen thermal denaturation by second harmonic generation microscopy

C.-S. Liao, Z.-Y. Zhuo, J.-Y. Yu, P.-H. G. Chao, S.-W. Chu

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756928 (2010) https://doi.org/10.1117/12.841204
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Collagen denaturation is of fundamental importance for clinical treatment. Conventionally, the denaturation process is quantified by the shrinkage of collagen fibers, but the underlying molecular origin has not been fully understood. Since second harmonic generation (SHG) is related to the molecular packing of the triple helix in collagen fibers, this nonlinear signal provides an insight of molecular dynamics during thermal denaturation. With the aid of SHG microscopy, we found a new step in collagen thermal denaturation process, de-crimp. During the de-crimp step, the characteristic crimp pattern of collagen fascicles disappeared due to the breakage of interconnecting bonds between collagen fibrils, while SHG intensity remained unchanged, suggesting the intactness of the triple helical molecules. At higher temperature, shrinkage is observed with strongly reduced SHG intensity, indicating denaturation at the molecular level.

Citation Download Citation

C.-S. Liao, Z.-Y. Zhuo, J.-Y. Yu, P.-H. G. Chao, and S.-W. Chu "In situ observation of collagen thermal denaturation by second harmonic generation microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756928 (26 February 2010); https://doi.org/10.1117/12.841204

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KEYWORDS

Collagen

Second-harmonic generation

Molecules

Polarization

Microscopy

Anisotropy

Harmonic generation

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