Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell

A. Alrifaiy; N. Bitaraf; O. Lindahl; K. Ramser

doi:10.1117/12.860478

27 August 2010 Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell

A. Alrifaiy, N. Bitaraf, O. Lindahl, K. Ramser

Author Affiliations +

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77622K (2010) https://doi.org/10.1117/12.860478
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

We present a new approach of combining Lab-on-a-chip technologies with optical manipulation technique for accurate investigations in the field of cell biology. A general concept was to develop and combine different methods to perform advanced electrophysiological investigations of an individual living cell under optimal control of the surrounding environment. The conventional patch clamp technique was customized by modifying the open system with a gas-tight multifunctional microfluidics system and optical trapping technique (optical tweezers). The system offers possibilities to measure the electrical signaling and activity of the neuron under optimum conditions of hypoxia and anoxia while the oxygenation state is controlled optically by means of a spectroscopic technique. A cellbased microfluidics system with an integrated patch clamp pipette was developed successfully. Selectively, an individual neuron is manipulated within the microchannels of the microfluidic system under a sufficient control of the environment. Experiments were performed to manipulate single yeast cell and red blood cell (RBC) optically through the microfluidics system toward an integrated patch clamp pipette. An absorption spectrum of a single RCB was recorded which showed that laser light did not impinge on the spectroscopic spectrum of light. This is promising for further development of a complete lab-on-a-chip system for patch clamp measurements.

Citation Download Citation

A. Alrifaiy, N. Bitaraf, O. Lindahl, and K. Ramser "Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77622K (27 August 2010); https://doi.org/10.1117/12.860478

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available