Cell imaging techniques based on digital image plane holography

Zhaoji Chen; Wendi Gong; Feifei Liu; Huaying Wang

doi:10.1117/12.870536

17 November 2010 Cell imaging techniques based on digital image plane holography

Zhaoji Chen, Wendi Gong, Feifei Liu, Huaying Wang

Proceedings Volume 7848, Holography, Diffractive Optics, and Applications IV; 78482Z (2010) https://doi.org/10.1117/12.870536
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

This paper has further studied the implementation methods and recording conditions of digital microscopic image plane holography (DMIPH). Two optical systems of DMIPH were built: one is recording hologram by using plane waves as reference light, the other is recording hologram by spherical reference light. Breast cancer cells and USAF resolution test target is used as tested samples in the experiment. Then the intensity distribution and three-dimensional shape information of the cells are got accurately. The experiment results show that DMIPH avoids the process of finding recording distance by using auto-focusing approach. The recording and reconstruction process of DMIPH is simple. Therefore DMIPH can be applied to the microscopic imaging of cells more effectively.

Citation Download Citation

Zhaoji Chen, Wendi Gong, Feifei Liu, and Huaying Wang "Cell imaging techniques based on digital image plane holography", Proc. SPIE 7848, Holography, Diffractive Optics, and Applications IV, 78482Z (17 November 2010); https://doi.org/10.1117/12.870536

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available