Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography

Narendran Sudheendran; Mohamed Mohamed; Mohamad G. Ghosn; Valery V. Tuchin; Kirill V. Larin

doi:10.1117/12.874991

11 February 2011 Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography

Narendran Sudheendran, Mohamed Mohamed, Mohamad G. Ghosn, Valery V. Tuchin, Kirill V. Larin

Author Affiliations +

Proceedings Volume 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV; 78892A (2011) https://doi.org/10.1117/12.874991
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In imaging of turbid biological samples using optical techniques, optical clearing methods can compensate for the lack of light penetration due to strong attenuation. The addition of optical clearing agents into scattering media increases the optical homogeneity of the sample and reduces its turbidity, allowing for the increased light penetration. In this study we investigated the extent of optical clearing in porcine skin by utilizing various concentrations of glucose solution. A goldplated mirror was fixed beneath the tissue and percentage clearing was determined by measuring the change in intensity of optical coherence tomography light returning from the mirror over time. A ratio of percentage clearing per tissue thickness for 10%, 30%, and 50% glucose was determined to be to be (4.7 ± 1.6%) mm^-1 (n = 6), (10.6 ± 2.0%) mm^-1 (n = 7), and (21.8 ± 2.2%) mm^-1 (n = 5), respectively. Although the extent of optical clearing in porcine skin was more significant for 50% glucose, the osmotic stress on the sample can cause considerable morphology change, thus a suitable concentration must be chosen for particular circumstances.

Citation Download Citation

Narendran Sudheendran, Mohamed Mohamed, Mohamad G. Ghosn, Valery V. Tuchin, and Kirill V. Larin "Assessment of tissue optical clearing as a function of glucose concentration using optical coherence tomography", Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 78892A (11 February 2011); https://doi.org/10.1117/12.874991

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Glucose

Optical clearing

Tissue optics

Mirrors

Tissues

Skin

Optical coherence tomography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years