Evaluation of autofocus measures for microscopy images of biopsy and cytology

R. Redondo; M. G. Bueno; J. C. Valdiviezo; R. Nava; G. Cristóbal; M. García; O. Déniz; B. Escalante-Ramírez

doi:10.1117/12.903314

2 November 2011 Evaluation of autofocus measures for microscopy images of biopsy and cytology

R. Redondo, M. G. Bueno, J. C. Valdiviezo, R. Nava, G. Cristóbal, M. García, O. Déniz, B. Escalante-Ramírez

Proceedings Volume 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World; 801194 (2011) https://doi.org/10.1117/12.903314
Event: International Commission for Optics (ICO 22), 2011, Puebla, Mexico

Abstract

An essential and indispensable component of automated microscopy is the automatic focusing system, which determines the in-focus position of a given field of view by searching for the maximal of an autofocus function over a range of z-axis positions. The autofocus function and its computation time are crucial to the accuracy and efficiency of the system. In this paper, we analyze and evaluate fifteen autofocus algorithms for biopsy and cytology microscopy images, ranging from the already well known methods to those proposed recently. Results have shown that there is a trade-off between computational cost and accuracy. Finally, the error committed by each of the algorithms is presented.

Citation Download Citation

R. Redondo, M. G. Bueno, J. C. Valdiviezo, R. Nava, G. Cristóbal, M. García, O. Déniz, and B. Escalante-Ramírez "Evaluation of autofocus measures for microscopy images of biopsy and cytology", Proc. SPIE 8011, 22nd Congress of the International Commission for Optics: Light for the Development of the World, 801194 (2 November 2011); https://doi.org/10.1117/12.903314

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