Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer

Nan Bai; Yanji Tang; Arun K. Bhunia; E. Daniel Hirleman; Euiwon Bae

doi:10.1117/12.884013

11 May 2011 Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer

Nan Bai, Yanji Tang, Arun K. Bhunia, E. Daniel Hirleman, Euiwon Bae

Author Affiliations +

Proceedings Volume 8027, Sensing for Agriculture and Food Quality and Safety III; 80270C (2011) https://doi.org/10.1117/12.884013
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

To experimentally analyze the morphological characteristics and to predict the resulting scattering patterns of different bacterial colonies, an optical morphology analyzer was constructed based on a laser confocal displacement meter to simultaneously obtain the optical properties of colonies. The profile data was accurately captured using the confocal laser triangulation technology and the transmitted light was collected by a photodiode circuit. The analog signals were read into a data acquisition board in parallel for off-line signal processing. This approach showed promising results for differentiation of micro-colonies in the range of 100~300 μm based on the morphological differences among different species using light scattering.

Citation Download Citation

Nan Bai, Yanji Tang, Arun K. Bhunia, E. Daniel Hirleman, and Euiwon Bae "Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer", Proc. SPIE 8027, Sensing for Agriculture and Food Quality and Safety III, 80270C (11 May 2011); https://doi.org/10.1117/12.884013

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