Dr. Arun K. Bhunia Profile

Dr. Arun K. Bhunia

at Purdue Univ

SPIE Involvement:

Author

Publications (25)

SPIE Journal Paper | 24 October 2016 Open Access

Reflected scatterometry for noninvasive interrogation of bacterial colonies

Huisung Kim, Iyll-Joon Doh, Jennifer Sturgis, Arun Bhunia, J. Paul Robinson, Euiwon Bae

JBO, Vol. 21, Issue 10, 107004, (October 2016) https://doi.org/10.1117/12.10.1117/1.JBO.21.10.107004

KEYWORDS: Blood, Diffraction, Scatterometry, Reflection, Bacteria, Photons, Opacity, Laser scattering, Backscatter, Image classification

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Proceedings Article | 3 June 2011 Paper

A distributed national network for label-free rapid identification of emerging pathogens

J. Paul Robinson, Bartek Rajwa, M. Murat Dundar, Euiwon Bae, Valery Patsekin, E. Daniel Hirleman, Ali Roumani, Arun Bhunia, J. Eric Dietz, V. Jo Davisson, John Thomas

Proceedings Volume 8018, 80180C (2011) https://doi.org/10.1117/12.884619

KEYWORDS: Pathogens, Organisms, Electrophoretic light scattering, Databases, Genetics, Light scattering, Scattering, Microorganisms, Microbiology, Bacteria

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Proceedings Article | 16 May 2011 Paper

Digital microbiology: detection and classification of unknown bacterial pathogens using a label-free laser light scatter-sensing system

Bartek Rajwa, M. Murat Dundar, Ferit Akova, Valery Patsekin, Euiwon Bae, Yanjie Tang, J. Eric Dietz, E. Daniel Hirleman, J. Paul Robinson, Arun Bhunia

Proceedings Volume 8029, 80290C (2011) https://doi.org/10.1117/12.884541

KEYWORDS: Classification systems, Pathogens, Library classification systems, Light scattering, Machine learning, Spectroscopy, Organisms, Laser scattering, Sensors, Statistical analysis

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Proceedings Article | 12 May 2011 Paper

Characterization of optical properties of bacterial micro-colonies via the comprehensive morphology analyzer

Nan Bai, Yanji Tang, Arun Bhunia, E. Daniel Hirleman, Euiwon Bae

Proceedings Volume 8027, 80270C (2011) https://doi.org/10.1117/12.884013

KEYWORDS: Transmittance, Light scattering, Scattering, Photodiodes, Laser scattering, Confocal microscopy, Optical properties, Code division multiplexing, Signal attenuation, Sensors

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SPIE Journal Paper | 1 July 2010 Open Access

Modeling light propagation through bacterial colonies and its correlation with forward scattering patterns

Euiwon Bae, Nan Bai, Amornrat Aroonnual, J. Paul Robinson, Arun Bhunia, E. Daniel Hirleman

JBO, Vol. 15, Issue 04, 045001, (July 2010) https://doi.org/10.1117/12.10.1117/1.3463003

KEYWORDS: Diffraction, Scattering, Light scattering, Bacteria, Wavefronts, Phase contrast, Confocal microscopy, Code division multiplexing, Laser scattering, Phase modulation

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Proceedings Article | 5 May 2009 Paper

Morphotypic analysis and classification of bacteria and bacterial colonies using laser light-scattering, pattern recognition, and machine-learning system

Bartek Rajwa, Murat Dundar, Valeri Patsekin, Karleigh Huff, Arun Bhunia, Murugesan Venkatapathi, Euiwon Bae, E. Daniel Hirleman, J. Paul Robinson

Proceedings Volume 7306, 73061A (2009) https://doi.org/10.1117/12.818589

KEYWORDS: Bacteria, Particles, Sensors, Classification systems, Light scattering, Image classification, Flow cytometry, Scatter measurement, Pathogens, Pattern recognition

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Proceedings Article | 28 April 2009 Paper

Development of a real-time system of monitoring bacterial colony growth and registering the forward-scattering pattern

Nan Bai, Euiwon Bae, Amornrat Aroonnual, Arun Bhunia, J. Paul Robinson, E. Daniel Hirleman

Proceedings Volume 7315, 73150Z (2009) https://doi.org/10.1117/12.818961

KEYWORDS: Bacteria, Scattering, Light scattering, Code division multiplexing, Scatterometry, Confocal microscopy, Convection, Safety, Microscopes, Real-time computing

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Proceedings Article | 28 April 2009 Paper

Prediction of the light scattering patterns from bacteria colonies by a time-resolved reaction-diffusion model and the scalar diffraction theory

Euiwon Bae, Nan Bai, Amornrat Aroonnual, Arun Bhunia, J. Paul Robinson, E. Daniel Hirleman

Proceedings Volume 7315, 73150A (2009) https://doi.org/10.1117/12.818855

KEYWORDS: Bacteria, Scattering, Mathematical modeling, Diffraction, Diffusion, Light scattering, Laser scattering, Modulators, Instrument modeling, Refractive index

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Proceedings Article | 22 February 2008 Paper

Phenotypic analysis of bacterial colonies using laser light scatter and pattern-recognition techniques

Bartek Rajwa, Bulent Bayraktar, Padmapriya Banada, Karleigh Huff, Euiwon Bae, E. Daniel Hirleman, Arun Bhunia, J. Paul Robinson

Proceedings Volume 6864, 68640S (2008) https://doi.org/10.1117/12.762366

KEYWORDS: Laser scattering, Bacteria, Classification systems, Detection and tracking algorithms, Light scattering, Pathogens, Image classification, Microorganisms, Matrices, Organisms

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Proceedings Article | 12 February 2008 Paper

Development and optimization of two-dimensional centering algorithm for bacterial rapid detection system using forward scattering

Euiwon Bae, Andry Lesmana, Arun Bhunia, J. Paul Robinson, E. Daniel Hirleman

Proceedings Volume 6849, 684905 (2008) https://doi.org/10.1117/12.763478

KEYWORDS: Scattering, Laser scattering, Cameras, Image segmentation, Image processing algorithms and systems, Algorithm development, Image processing, Imaging systems, Beam splitters, Detection and tracking algorithms

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SPIE Journal Paper | 1 January 2008 Open Access

Analysis of time-resolved scattering from macroscale bacterial colonies

Euiwon Bae, Padmapriya Banada, Karleigh Huff, Arun Bhunia, J. Paul Robinson, E. Daniel Hirleman

JBO, Vol. 13, Issue 01, 014010, (January 2008) https://doi.org/10.1117/12.10.1117/1.2830655

KEYWORDS: Scattering, Laser scattering, Light scattering, Phase modulation, Microscopes, Phase contrast, Bacteria, Biological research, Modulation, Pathogens

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Proceedings Article | 26 April 2007 Paper

Noninvasive forward-scattering system for rapid detection, characterization, and identification of bacterial colonies

Bartek Rajwa, Bulent Bayraktar, Padmapriya Banada, Karleigh Huff, Euiwon Bae, E. Daniel Hirleman, Arun Bhunia, J. Paul Robinson

Proceedings Volume 6554, 65540N (2007) https://doi.org/10.1117/12.722200

KEYWORDS: Pathogens, Laser scattering, Organisms, Computing systems, Bacteria, Feature extraction, Detection and tracking algorithms, Image classification, Contamination, Solids

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Proceedings Article | 16 February 2007 Paper

Quantification of morphology of bacterial colonies using laser scatter measurements and solid element optical modeling

Silas Leavesley, Bülent Bayraktar, Murugesan Venkatapathi, E. Dan Hirleman, Arun Bhunia, J. Paul Robinson, Richard Hassler, Linda Smith, Bartek Rajwa

Proceedings Volume 6446, 64460E (2007) https://doi.org/10.1117/12.701224

KEYWORDS: Solids, Light scattering, Scattering, Scatter measurement, Optical properties, Geometrical optics, Refractive index, Laser scattering, Optical components, Pathogens

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Proceedings Article | 23 October 2006 Paper

Fiber optic biosensor employing Alexa-Fluor conjugated antibodies for detection of Escherichia coli O157:H7 and Shiga-like toxins

Shu-I Tu, Tao Geng, Joe Uknalis, Arun Bhunia

Proceedings Volume 6381, 638106 (2006) https://doi.org/10.1117/12.686316

KEYWORDS: Waveguides, Bacteria, Fiber optics, Biosensors, Signal detection, Luminescence, Molecules, Fiber optics sensors, Pathogens, Analytical research

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Proceedings Article | 23 October 2006 Paper

Biophysical modeling of time-resolved forward scattering by Listeria colonies

Euiwon Bae, Padmapriya Banada, Arun Bhunia, E. Daniel Hirleman

Proceedings Volume 6381, 638107 (2006) https://doi.org/10.1117/12.686406

KEYWORDS: Scattering, Light scattering, Laser scattering, Phase contrast, Bacteria, Phase modulation, Time metrology, Pathogens, Gaussian beams, Diffraction

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We have developed a detection system and associated protocol based on optical forward scattering where the bacterial colonies of various species and strains growing on solid nutrient surfaces produced unique scatter signatures. The aim of the present investigation was to develop a bio-physical model for the relevant phenomena. In particular, we considered time-varying macroscopic morphological properties of the growing colonies and modeled the scattering using scalar diffraction theory. For the present work we performed detailed studies with three species of Listeria; L. innocua, L. monocytogenes, and L. ivanovii. The baseline experiments involved cultures grown on brain heart infusion (BHI) agar and the scatter images were captured every six hours for an incubation period of 42 hours. The morphologies of the colonies were studied by phase contrast microscopy, including measurement of the diameter of the colony. Growth curves, represented by colony diameter as a function of time, were compared with the time-evolution of scattering signatures. Similar studies were carried out with L. monocytogenes grown on different substrates. Non-dimensionalizing incubation time in terms of the time to reach stationary phase was effective in reducing the dimensionality of the model. Bio-physical properties of the colony such as diameter, bacteria density variation, surface curvature/profile, and transmission coefficient are important parameters in predicting the features of the forward scattering signatures. These parameters are included in a baseline model that treats the colony as a concentric structure with radial variations in phase modulation. In some cases azimuthal variations and random phase inclusions were included as well. The end result is a protocol (growth media, incubation time and conditions) that produces reproducible and distinguishable scatter patterns for a variety of harmful food borne pathogens in a short period of time. Further, the bio-physical model we developed is very effective in predicting the dominant features of the scattering signatures required by the identification process and will be effective for informing further improvements in the instrumentation.

Proceedings Article | 23 October 2006 Paper

Noninvasive forward-scattering system for rapid detection, characterization, and identification of Listeria colonies: image processing and data analysis

Bartek Rajwa, Bulent Bayraktar, Padmapriya Banada, Karleigh Huff, Euiwon Bae, E. Daniel Hirleman, Arun Bhunia, J. Paul Robinson

Proceedings Volume 6381, 638105 (2006) https://doi.org/10.1117/12.686298

KEYWORDS: Laser scattering, Pathogens, Image classification, Feature extraction, Data analysis, Classification systems, Image processing, Feature selection, Machine learning, Agriculture

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SPIE Journal Paper | 1 May 2006 Open Access

Feature extraction from light-scatter patterns of Listeria colonies for identification and classification

Bulent Bayraktar, Padmapriya Banada, Edwin Hirleman, Arun Bhunia, J. Paul Robinson, Bartlomiej Rajwa

JBO, Vol. 11, Issue 03, 034006, (May 2006) https://doi.org/10.1117/12.10.1117/1.2203987

KEYWORDS: Pathogens, Feature extraction, Image classification, Bacteria, Laser scattering, Principal component analysis, Semiconductor lasers, Error analysis, Visualization, Feature selection

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Proceedings Article | 25 February 2006 Paper

Bacterial phenotype identification using Zernike moment invariants

Bulent Bayraktar, Padmapriya Banada, E. Daniel Hirleman, Arun Bhunia, J. Paul Robinson, Bartek Rajwa

Proceedings Volume 6080, 60800V (2006) https://doi.org/10.1117/12.647879

KEYWORDS: Principal component analysis, Pathogens, Light scattering, Bacteria, Feature extraction, Image classification, Detection and tracking algorithms, Analytical research, Contamination, Scattering

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Proceedings Article | 9 November 2005 Paper

A portable cell-based optical detection device for rapid detection of Listeria and Bacillus toxins

Pratik Banerjee, Padmapriya Banada, Jenna Rickus, Mark Morgan, Arun Bhunia

Proceedings Volume 5996, 599602 (2005) https://doi.org/10.1117/12.630793

KEYWORDS: Collagen, Pathogens, Aluminium phosphide, Sensors, Bacteria, Proteins, Matrices, Safety, Agriculture, Biosensors

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Proceedings Article | 8 November 2005 Paper

Microfluidic pretreatment of bacterial cells for analysis of intracellular contents

Hsiang-Yu Wang, Chang Lu, Padmapriya Banada, Balamurugan Jagadeesan, Arun Bhunia

Proceedings Volume 5996, 599603 (2005) https://doi.org/10.1117/12.630887

KEYWORDS: Microfluidics, Chemical analysis, Green fluorescent protein, Luminescence, Lithography, Proteins, Microscopy, Biological research, Electrodes, Fluorescence spectroscopy

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Proceedings Article | 30 March 2004 Paper

Optical immunosensors for detection of Listeria monocytogenes and Salmonella enteritidis from food

Arun Bhunia, Tao Geng, Amanda Lathrop, Angela Valadez, Mark Morgan

Proceedings Volume 5271, (2004) https://doi.org/10.1117/12.515900

KEYWORDS: Sensors, Fiber optics sensors, Proteins, Mirrors, Pathogens, Fiber optics, Scanning electron microscopy, Surface plasmons, Microorganisms, Bacteria

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Proceedings Article | 13 March 2001 Paper

Identification and characterization of bacteria on surfaces using light scattering

Brent Nebeker, Benjamin Buckner, E. Dan Hirleman, Amanda Lathrop, Arun Bhunia

Proceedings Volume 4206, (2001) https://doi.org/10.1117/12.418733

KEYWORDS: Light scattering, Scattering, Bacteria, Laser scattering, Scatter measurement, Pathogens, Semiconductors, Silicon, Microorganisms, Particles

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Proceedings Article | 13 March 2001 Paper

Sequential disinfection of E. coli O157:H7 on shredded lettuce leaves by aqueous chlorine dioxide, ozonated water, and thyme essential oil

Nepal Singh, Rakesh Singh, Arun Bhunia, Richard Stroshine, James Simon

Proceedings Volume 4206, (2001) https://doi.org/10.1117/12.418725

KEYWORDS: Chlorine, Ozone, Chlorine gas, Pathogens, Control systems, Microorganisms, Bacteria, Organic materials, Statistical analysis, Oxidation

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Proceedings Article | 13 March 2001 Paper

Impedance spectroscopy and biochip sensor for detection of Listeria monocytogenes

Arun Bhunia, Ziad Jaradat, Kristen Naschansky, Mindy Shroyer, Mark Morgan, Rafael Gomez, Rashid Bashir, Mike Ladisch

Proceedings Volume 4206, (2001) https://doi.org/10.1117/12.418740

KEYWORDS: Fiber optics, Pathogens, Biosensors, Electrodes, Bacteria, Dielectric spectroscopy, Luminescence, Sensors, Tissues, Microsensors

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Proceedings Article | 13 March 2001 Paper

Light-emission-based biosensor for detection of food pathogens: a review

Ashutosh Singh, Rakesh Singh, Arun Bhunia

Proceedings Volume 4206, (2001) https://doi.org/10.1117/12.418723

KEYWORDS: Biosensors, Pathogens, Mirrors, Metals, Waveguides, Biological research, Surface plasmons, Sensors, Diffraction gratings, Refractive index

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Showing 5 of 25 publications

Conference Committee Involvement (16)

Sensing for Agriculture and Food Quality and Safety XI

16 April 2019 | Baltimore, MD, United States

Sensing for Agriculture and Food Quality and Safety X

17 April 2018 | Orlando, FL, United States

Sensing for Agriculture and Food Quality and Safety IX

13 April 2017 | Anaheim, CA, United States

Sensing for Agriculture and Food Quality and Safety VIII

20 April 2016 | Baltimore, MD, United States

Sensing for Agriculture and Food Quality and Safety VII

21 April 2015 | Baltimore, MD, United States

Sensing for Agriculture and Food Quality and Safety VI

6 May 2014 | Baltimore, MD, United States

Sensing for Agriculture and Food Quality and Safety V

30 April 2013 | Baltimore, Maryland, United States

Sensing for Agriculture and Food Quality and Safety IV

24 April 2012 | Baltimore, Maryland, United States

Sensing for Agriculture and Food Quality and Safety III

26 April 2011 | Orlando, Florida, United States

Sensing for Agriculture and Food Quality and Safety II

6 April 2010 | Orlando, Florida, United States

Sensing for Agriculture and Food Quality and Safety

14 April 2009 | Orlando, Florida, United States

Special Sessions on Food Safety, Visual Analytics, Resource Restricted Embedded and Sensor Networks, and 3D Imaging and Display

17 March 2008 | Orlando, Florida, United States

Optics for Natural Resources, Agriculture, and Foods II

10 September 2007 | Boston, MA, United States

Optics for Natural Resources, Agriculture, and Foods

3 October 2006 | Boston, Massachusetts, United States

Optical Sensors and Sensing Systems for Natural Resources and Food Safety and Quality

23 October 2005 | Boston, MA, United States

Nondestructive Sensing for Food Safety, Quality, and Natural Resources

26 October 2004 | Philadelphia, Pennsylvania, United States

Showing 5 of 16 Conference Committees

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