Quantitative phase microscopy for the study of bone cells on multiple roughness surfaces

Jesús González-Laprea; Ana H. Márquez; Karem Noris-Suarez; Rafael Escalona

doi:10.1117/12.928437

13 September 2012 Quantitative phase microscopy for the study of bone cells on multiple roughness surfaces

Jesús González-Laprea, Ana H. Márquez, Karem Noris-Suarez, Rafael Escalona

Proceedings Volume 8494, Interferometry XVI: Applications; 84940Q (2012) https://doi.org/10.1117/12.928437
Event: SPIE Optical Engineering + Applications, 2012, San Diego, California, United States

Abstract

We present a phase shifting interferometry system for the study of the early adhesion process for osteoblast-like cells, through an interference microscope. Optical phase maps from the cells are obtained experimentally as a function of cell adhesion time. The process is carried out on surfaces of metallic materials relevant to the development of bone implants. The surfaces were subjected to various levels of mechanical polishing and their roughness was measured using the same experimental technique mentioned before. Morphological changes of the cell can be measured over their optical phase maps while the cell adhesion process is accomplished. The experimental technique shows a suitable feature as to the observed time scale, and also shows a high stockiness and precision for the determination of the optical phase.

Citation Download Citation

Jesús González-Laprea, Ana H. Márquez, Karem Noris-Suarez, and Rafael Escalona "Quantitative phase microscopy for the study of bone cells on multiple roughness surfaces", Proc. SPIE 8494, Interferometry XVI: Applications, 84940Q (13 September 2012); https://doi.org/10.1117/12.928437

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