Highly fluorescent semiconducting polymer dots for single-molecule imaging and biosensing

Wei Sun; Jiangbo Yu; Fangmao Ye; Yu Rong; Daniel T. Chiu

doi:10.1117/12.2028187

11 September 2013 Highly fluorescent semiconducting polymer dots for single-molecule imaging and biosensing

Wei Sun, Jiangbo Yu, Fangmao Ye, Yu Rong, Daniel T. Chiu

Proceedings Volume 8812, Biosensing and Nanomedicine VI; 881205 (2013) https://doi.org/10.1117/12.2028187
Event: SPIE NanoScience + Engineering, 2013, San Diego, California, United States

Abstract

This paper describes the preparation of semiconducting polymer dots (Pdots) and their application for single-molecule imaging and biosensing. The Pdots possessed high fluorescence brightness, with a 16 nm Pdot being ~9 times brighter than 13 nm Quantum Dots (Qdots). The surface of Pdots was successfully conjugated with streptavidin, which made Pdots suitable for specific subcellular labeling and targeting. The interior composition of Pdots was also successfully modified, through which the Pdots obtained additional functionalities. We demonstrated the utility of gold nanoparticle embedded Pdots in dual-modality imaging. We also demonstrated that Rhodamine B embedded Pdots were able to function as ratiometric temperature sensor in live-cell imaging mode.

Citation Download Citation

Wei Sun, Jiangbo Yu, Fangmao Ye, Yu Rong, and Daniel T. Chiu "Highly fluorescent semiconducting polymer dots for single-molecule imaging and biosensing", Proc. SPIE 8812, Biosensing and Nanomedicine VI, 881205 (11 September 2013); https://doi.org/10.1117/12.2028187

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