Capturing CD4 cells using a functionalized circular microfluidic device and glutaraldehyde as biolinker for tuberculosis detection and diagnosis

Yeu-Farn Shih; Nien-Tsu Huang; Chih-Kung Lee

doi:10.1117/12.2078693

5 March 2015 Capturing CD4 cells using a functionalized circular microfluidic device and glutaraldehyde as biolinker for tuberculosis detection and diagnosis

Yeu-Farn Shih, Nien-Tsu Huang, Chih-Kung Lee

Author Affiliations +

Proceedings Volume 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII; 93200U (2015) https://doi.org/10.1117/12.2078693
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

It is estimated that about one-third of the world’s population has already been infected by tuberculosis. Mycobacterium tuberculosis, in general, can result in an active case of tuberculosis in approximately 5%-10% of those who suffer from latent tuberculosis and the chance of becoming ill is the highest within one of year of getting the disease. Although a newly developed methods called interferon gamma release assay (IGRA) can monitor CD4 cells secreted cytokine to diagnose tuberculosis (TB) condition. However, it is difficult to count total numbers of cytokine secreted CD4 cells, which make the diagnosis less accurate. Therefore, we develop a functionalized polydimethylsiloxane (PDMS) device using glutaraldehyde to capture CD4 cells. To enhance the capture efficiency, we use COMSOL simulation to optimize the arrangement of PDMS micro pillars to make cells uniformly distributed in the device. Our preliminary data showed the microfluidic configuration in a circular shape with HCP patterned micro pillars turned 30 degrees offers the highest cell capture rate.

Citation Download Citation

Yeu-Farn Shih, Nien-Tsu Huang, and Chih-Kung Lee "Capturing CD4 cells using a functionalized circular microfluidic device and glutaraldehyde as biolinker for tuberculosis detection and diagnosis", Proc. SPIE 9320, Microfluidics, BioMEMS, and Medical Microsystems XIII, 93200U (5 March 2015); https://doi.org/10.1117/12.2078693

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