Multilayer based lab-on-a-chip-systems for substance testing

Frank Sonntag; Stefan Grünzner; Florian Schmieder; Mathias Busek; Udo Klotzbach; Volker Franke

doi:10.1117/12.2083100

12 March 2015 Multilayer based lab-on-a-chip-systems for substance testing

Frank Sonntag, Stefan Grünzner, Florian Schmieder, Mathias Busek, Udo Klotzbach, Volker Franke

Proceedings Volume 9351, Laser-based Micro- and Nanoprocessing IX; 93510C (2015) https://doi.org/10.1117/12.2083100
Event: SPIE LASE, 2015, San Francisco, California, United States

Abstract

An integrated technology chain for laser-microstructuring and bonding of polymer foils for fast, flexible and low-cost manufacturing of multilayer lab-on-a-chip devices especially for complex cell and tissue culture applications, which provides pulsatile fluid flow within physiological ranges at low media-to-cells ratio, was developed and established. Initially the microfluidic system is constructively divided into individual layers which are formed by separate foils or plates. Based on the functional boundary conditions and the necessary properties of each layer the corresponding foils and plates are chosen. In the third step the foils and plates are laser microstructured and functionalized from both sides. In the fourth and last manufacturing step the multiple plates and foils are joined using thermal diffusion bonding. Membranes for pneumatically driven valves and micropumps where bonded via chemical surface modification. Based on the established lab-on-a-chip platform for perfused cell-based assays, a multilayer microfluidic system with two parallel connected cell culture chambers was successfully implemented.

Citation Download Citation

Frank Sonntag, Stefan Grünzner, Florian Schmieder, Mathias Busek, Udo Klotzbach, and Volker Franke "Multilayer based lab-on-a-chip-systems for substance testing", Proc. SPIE 9351, Laser-based Micro- and Nanoprocessing IX, 93510C (12 March 2015); https://doi.org/10.1117/12.2083100

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