Monitoring hemodynamics and oxygenation of the kidney in rats by a combined near-infrared spectroscopy and invasive probe approach

Dirk Grosenick; Kathleen Cantow; Karen Arakelyan; Heidrun Wabnitz; Bert Flemming; Angela Skalweit; Mechthild Ladwig; Rainer Macdonald; Thoralf Niendorf; Erdmann Seeliger

doi:10.1117/12.2183417

16 July 2015 Monitoring hemodynamics and oxygenation of the kidney in rats by a combined near-infrared spectroscopy and invasive probe approach

Dirk Grosenick, Kathleen Cantow, Karen Arakelyan, Heidrun Wabnitz, Bert Flemming, Angela Skalweit, Mechthild Ladwig, Rainer Macdonald, Thoralf Niendorf, Erdmann Seeliger

Author Affiliations +

Proceedings Volume 9538, Diffuse Optical Imaging V; 95380L (2015) https://doi.org/10.1117/12.2183417
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

We have developed a hybrid approach to investigate the dynamics of perfusion and oxygenation in the kidney of rats under pathophysiologically relevant conditions. Our approach combines near-infrared spectroscopy to quantify hemoglobin concentration and oxygen saturation in the renal cortex, and an invasive probe method for measuring total renal blood flow by an ultrasonic probe, perfusion by laser-Doppler fluxmetry, and tissue oxygen tension via fluorescence quenching. Hemoglobin concentration and oxygen saturation were determined from experimental data by a Monte Carlo model. The hybrid approach was applied to investigate and compare temporal changes during several types of interventions such as arterial and venous occlusions, as well as hyperoxia, hypoxia and hypercapnia induced by different mixtures of the inspired gas. The approach was also applied to study the effects of the x-ray contrast medium iodixanol on the kidney.

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Dirk Grosenick, Kathleen Cantow, Karen Arakelyan, Heidrun Wabnitz, Bert Flemming, Angela Skalweit, Mechthild Ladwig, Rainer Macdonald, Thoralf Niendorf, and Erdmann Seeliger "Monitoring hemodynamics and oxygenation of the kidney in rats by a combined near-infrared spectroscopy and invasive probe approach", Proc. SPIE 9538, Diffuse Optical Imaging V, 95380L (16 July 2015); https://doi.org/10.1117/12.2183417

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KEYWORDS

Oxygen

Tissues

Kidney

Near infrared spectroscopy

Blood

Hypoxia

X-rays

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