A novel measurement method of microorganism growth by tunable diode laser-absorption spectroscopy

Jindong Xiang; Jie Shao; Chaofu Ying; Liming Wang; Jie Guo

doi:10.1117/12.2181843

4 May 2015 A novel measurement method of microorganism growth by tunable diode laser-absorption spectroscopy

Jindong Xiang, Jie Shao, Chaofu Ying, Liming Wang, Jie Guo

Proceedings Volume 9543, Third International Symposium on Laser Interaction with Matter; 954315 (2015) https://doi.org/10.1117/12.2181843
Event: Third International Symposium on Laser Interaction with Matter, 2014, Jiangsu, China

Abstract

The objective of this work was to attain essential parameters by using a Gompertz model that employed a new approach of wavelength modulation spectroscopy (WMS) to describe the microorganism growth. The measurement method of WMS introduces noninvasive technique instead of complicated invasive microorganism operation analysis and quickly obtains the accurate real-time measurement results. By using the WMS measurement, the specific growth curve of microorganism growth clearly displayed every three minute, which has characteristics of high sensitivity, high spectral resolution, fast time response and overcomes the randomness and error operation of traditional analysis methods. The measurement value of BF and AF in the range of 1.008 to 1.043 and the lower MSE showed that Gompertz model can fit the data well and be capable of describing bacteria growth rate and lag time. The results of experiment data suggested that the specific growth rate of microorganism depends on the temperature. With the increase of temperature ranging from 25 °C to 42 °C , the lag time of bacteria growth has been shortened. And the suitable temperature of bacteria growth is about 37 °C . Judging from the growth rate of microorganisms, we can identify the microbial species, not only to improve the precision and efficiency, but also to provides a rapidly sensitive way for microbial detection. The lag time of microorganism growth also provides a great application prospect for shelf life of the food safety.

Citation Download Citation

Jindong Xiang, Jie Shao, Chaofu Ying, Liming Wang, and Jie Guo "A novel measurement method of microorganism growth by tunable diode laser-absorption spectroscopy", Proc. SPIE 9543, Third International Symposium on Laser Interaction with Matter, 954315 (4 May 2015); https://doi.org/10.1117/12.2181843

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
12 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Microorganisms

Bacteria

Data modeling

Spectroscopy

Laser spectroscopy

Atrial fibrillation

Modulation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years