High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion

Xiang Lu; Rainer Heintzmann; Ulrich Leischner

doi:10.1117/12.2190823

23 September 2015 High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion

Xiang Lu, Rainer Heintzmann, Ulrich Leischner

Author Affiliations +

Proceedings Volume 9626, Optical Systems Design 2015: Optical Design and Engineering VI; 96262I (2015) https://doi.org/10.1117/12.2190823
Event: SPIE Optical Systems Design, 2015, Jena, Germany

Abstract

Light sheet microscopy is a microscopy technique characterized by an illumination from the side, perpendicular to the direction of observation. While this is often used and easy to implement for imaging samples with water-immersion, the application in combination with oil-immersion is less often used and requires a specific optimization. In this paper we present our design of a light-sheet illumination optical system with a ~1μm illumination thickness, a long working distance through the immersion oil, and including a focusing system allowing for moving the focus-spot of the lightsheet laterally through the field of view. This optical design allows for the acquisition of fluorescence images in 3D with isotropic resolution of below 1 micrometer of whole-mount samples with a size of ~1mm diameter. This technique enables high-resolution insights in the 3D structure of biological samples, e.g. for research of insect anatomy or for imaging of biopsies in medical diagnostics.

Citation Download Citation

Xiang Lu, Rainer Heintzmann, and Ulrich Leischner "High-resolution light-sheet microscopy: a simulation of an optical illumination system for oil immersion", Proc. SPIE 9626, Optical Systems Design 2015: Optical Design and Engineering VI, 96262I (23 September 2015); https://doi.org/10.1117/12.2190823

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